Skip to main content

CRISPR/Cas Tools

List of software platforms and bioinformatics tools built to facilitate the design of guide RNAs (gRNAs) for use with the CRISPR/Cas system.


 
Tool Name External Link Provider
Synthego Design Tool Webserver Synthego
CASTING Webserver Caagle
Benchling CRISPR gRNA Design Webserver Benchling
Breaking-Cas Webserver Spanish National Center for Biotechnology
Cas-OFFinder Webserver Source code Seoul National University
CRISPOR Webserver Source code University of California, Santa Cruz TEFOR
CCTop Webserver University of Heidelberg
CHOPCHOP Webserver Harvard University
CHOPCHOP v2 Webserver Source code University of Bergen
COD Webserver Dayong Guo
CRISPR Configurator & Specificity Tool Webserver Specificity Tool Dharmacon, Inc.
CRISPR Design Webserver Zhang Lab, MIT
CRISPRdirect Webserver Database Center for Life Science (DBCLS)
CRISPR gRNA Design Tool Webserver DNA2.0
CRISPR LifePipe Webserver Life and Soft
CRISPRseek Source code Bioconductor
DESKGEN Webserver Desktop Genetics
Genedata Selector Webserver Genedata
Geneious CRISPR Site Finder Geneious Geneious
GT-Scan Webserver CSIRO & EMBL-ABR
Off-Spotter Webserver Source code Remote batch submission Thomas Jefferson University
sgRNA Designer Webserver Source code Broad Institute

Comments

Most Viewed Post

How to keep chain ID / IDs in GROMACS?

In GROMACS , while converting pdb file (monomer or multimer) into .gro file, it do not preserve the chain ID information. Due to the lack of chain ID information, pdb file retrieved from .gro file at any stage of the simulation has missing chain IDs and pdb file can not be visualized properly in PYMOL / RASMOL . There are two ways to convert .gro file into .pdb Lets say your protein name is xyz.pdb 1] gmx editconf -f xyz.gro -o xyz.pdb 2] gmx trjconv -f  xyz.gro -o xyz.pdb -s xyz.tpr Only ' trjconv ' will retrieve the chain ID information for all the chains. and not ' editconf '. If you have monomer protein and wish to assign any chain ID then following command will be of your interest: gmx editconf -f xyz.gro -o xyz.pdb -label [ chain-ID ]

Python : Turtle tree

Turtle module can be used to draw some very nice patterns in Python. Following are some examples with code. ==================== import turtle import random t = turtle.Turtle( shape = "circle" ) t.lt( 90 ) lv = 14 l = 120 s = 30 t.color( 'indigo' ) t.width(lv) t.penup() t.bk(l) t.pendown() t.fd(l) def draw_tree ( l , level ): width = t.width() # save the current pen width t.width(width * 3.0 / 4.0 ) # narrow the pen width l = 3.0 / 4.0 * l #t.color(R,G,B) #provide the RGB numbers t.color(random.random(), random.random(), random.random()) t.lt(s) t.fd(l) if level < lv: draw_tree(l, level + 1 ) t.color(random.random(), random.random(), random.random()) t.bk(l) t.rt( 2 * s) t.fd(l) if level < lv: draw_tree(l, level + 1 ) t.color(random.random(), random.random(), random.random()) t.bk(l) t.lt(s) t.width(width) # restore the previous pen width t.speed( "fastest" ) draw_tree(l, 5 ) turtle.done() ===========...

How to use MODELLER to build DIMER homology model with ligand?

How to use MODELLER to build DIMER homology model with ligand? Procedure:     Get Fasta sequence from UNIPROT database.     Predict sequence alignment from HHPRED     Prepare INPUT files for MODELLER     model-dimer.py (Click to download) ############### from modeller import * from modeller.automodel import * #from modeller import soap_protein_od env = environ() env.io.hetatm = True a = automodel(env, alnfile='TvLDH-1bdm.ali',               knowns='1bdm',               sequence='TvLDH',               assess_methods=(assess.DOPE,                            ...

GNUPLOT: How to draw trend line?

How to draw trend line in the GNUPLOT? If you like to plot graphs in gnuplot and dont know how to plot trendline then here you are. Follow the steps mentioned below... 1. You should have a files with X and Y values 2. Open GNUPLOT (Operating system dosen't change anything here. It works on all systems) 3. Type the command in the gnuplot terminal Lets say I have a file for eg. '1.txt' p '1.txt' u 1:2 w d title '', '1.txt' u 1:2 smooth acsplines title '1.txt' OR p '1.txt' u 1:2 w d title '', '1.txt' u 1:2 smooth bezier title '1.txt' It will plot as below...

Plagarism Checker

Plagiarism is a serious academic misconduct. Whether you are a student writing a college essay, a teacher reviewing a student’s submission, or just someone who works extensively with content, it is important to ensure that the content is not plagiarized. Following are some resources to check Plagiarism. http://www.plagscan.com https://www.plagramme.com/   http://www.plagiarisma.net/fr/# http://www.scanmyessay.com http://www.plagtracker.com http://www.duplichecker.com http://www.smallseotools.com/plagiarism-checker http://www.plagium.com/fr/detecteurdeplagiat http://www.paperrater.com/plagiarism_checker http://www.copyleaks.com http://www.plagiarismchecker.com http://www.quetext.com http://plagiarismdetector.net http://www.solidseotools.com/plagiarism-checker http://www.dustball.com/cs/plagiarism.checker http://www.articlechecker.com http://www.plagiarismcheck.org

Science News

Enter your email address:

PhD Vacancy Bioinformatics

PhD Vacancy Chemoinformatics